Reassociation kinetics-based approach for partial genome sequencing of the cattle tick, Rhipicephalus (Boophilus) microplus

Journal Article
Published on
bySpringer

Abstract

The size and repetitive nature of the Rhipicephalus microplus genome makes obtaining a full genome sequence fiscally and technically problematic. To selectively obtain gene-enriched regions of this tick's genome, Cot filtration was performed, and Cot-filtered DNA was sequenced via 454 FLX pyrosequencing.

Citation

Guerrero, F.D.; Moolhuijzen, P.; Peterson, D.G.; Bidwell, S.; Caler, E.; Bellgard, M.; Nene, V.M.; Djikeng, A. 2010. Reassociation kinetics-based approach for partial genome sequencing of the cattle tick, Rhipicephalus (Boophilus) microplus. BMC Genomics 11: 374.

Authors

  • Guerrero, F.D.
  • Moolhuijzen, P.
  • Peterson, D.G.
  • Bidwell, S.
  • Caler, E.
  • Bellgard, M.
  • Nene, Vishvanath M.
  • Djikeng, Appolinaire

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